Analysis Note

ControlHEK293 cell lysate

Application

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Eizema, 2007).

Anti-SERCA2 Antibody, clone IID8 is an antibody against SERCA2 for use in WB, IC, IH.

Research CategoryCell Structure

Research Sub CategoryCytoskeletonCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs) mediate Ca2+ uptake into intracellular stores. SERCA mediated Ca2+ uptake induces and maintains muscular relaxation. SERCA1 is expressed in fast-twitch muscles, whereas SERCA2 is expressed in cardiac and slow-twitch muscles. SERCA2 plays an important part in regulating cardiac contractile function.

Immunogen

Epitope: Unknown

Purified canine cardiac sarcoplasmic reticulum.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.5 µg/ml of the antibody detected SERCA2 in 10 µg of HEK293 cell lysate.

Specificity

The antibody recognizes SERCA2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 110 kDa